pdk1 polyclonal antibody  (Proteintech)

Journal: iScience

Article Title: Identification and validation of key PANoptosis-related genes via integrative machine learning and single-cell sequencing in AILI

doi: 10.1016/j.isci.2026.115183

Figure Lengend Snippet: Expression and functional exploration of the key genes in single-cell sequencing data (A) The UMAP plot shows the total sample composition, tissue sources, and cell subtypes. (B) The stacked graph shows the proportion of each type of cell in the control group and the AILI group. (C) Bubble plots of marker gene expression demonstrating the accuracy of the cell annotations. (D) Bubble chart showing the expression of Cdkn1a and Pdk1 in various cells in the control group. (E) Bubble chart showing the expression of Cdkn1a and Pdk1 in various cells in the AILI group. (F) Circle plot and heatmap showing the cell communication weights and numbers of all cell subtypes. (G–J) Receptor‒ligand communication weights between AILI and control samples.

Article Snippet: PDK1 Polyclonal antibody , Proteintech , Cat No.18262-1-AP; RRID: AB_10598310.

Techniques: Expressing, Functional Assay, Single Cell, Sequencing, Control, Marker, Gene Expression

Journal: iScience

Article Title: Identification and validation of key PANoptosis-related genes via integrative machine learning and single-cell sequencing in AILI

doi: 10.1016/j.isci.2026.115183

Figure Lengend Snippet: Validation of key PANoptosis-related genes in animal models (A) H&E staining of liver tissues from WT and AILI mice (scale bars, 100 μm; n = 5). (B) mRNA expression of Cdkn1a and Pdk1 by RT-qPCR. (C–F) Correlation analyses between hepatic Cdkn1a and Pdk1 mRNA levels and serum ALT and AST levels at 24 h after AILI. (G) Detection and statistical analysis of key PANoptosis-related gene and marker protein expression in liver tissues from WT and AILI mice. (H) Immunohistochemical staining for P21 and PDK1 in liver tissues from WT and AILI mice (scale bars, 50 μm; n = 5). All the data are presented as the means ± SDs. One-way ANOVA with Tukey’s test and a two-tailed Student’s t test were used for statistical analysis. Spearman’s rank correlation was used to assess the associations between relative mRNA expression levels of Cdkn1a and Pdk1 and serum ALT and AST levels. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: PDK1 Polyclonal antibody , Proteintech , Cat No.18262-1-AP; RRID: AB_10598310.

Techniques: Biomarker Discovery, Staining, Expressing, Quantitative RT-PCR, Marker, Immunohistochemical staining, Two Tailed Test

Journal: iScience

Article Title: Identification and validation of key PANoptosis-related genes via integrative machine learning and single-cell sequencing in AILI

doi: 10.1016/j.isci.2026.115183

Figure Lengend Snippet: Pdk1 knockdown exacerbates AILI by promoting PANoptosis in vivo Mice were assigned to four groups: control, sh- NC , APAP, and sh- Pdk1 + APAP. (A) Schematic illustration of the experimental design. (B) Western blot analysis confirming efficient knockdown of PDK1 protein in liver tissues. (C) Hematoxylin and eosin (H&E) staining of liver sections and quantification of hepatic necrotic areas. Scale bars, 100 μm; n = 5. (D–E) Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels. (F) Serum levels of TNF-α, IL-1β, and IL-6 were measured by ELISA. (G) Western blot analysis and quantification of PANoptosis marker proteins in liver tissues. (H–K) Representative immunofluorescence staining of liver sections showing albumin (ALB, green) and PANoptosis marker proteins (ZBP1, p -MLKL, cleaved caspase-1, and cleaved caspase-3; red). Scale bars, 20 μm; n = 5. Data are presented as mean ± SD. One-way ANOVA with Tukey’s test and a two-tailed Student’s t test were used for statistical analysis. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: PDK1 Polyclonal antibody , Proteintech , Cat No.18262-1-AP; RRID: AB_10598310.

Techniques: Knockdown, In Vivo, Control, Western Blot, Staining, Enzyme-linked Immunosorbent Assay, Marker, Immunofluorescence, Two Tailed Test